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Integrative Cancer Biology @ Cancer Research UK Cambridge Institute

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Andrew Holding

Andrew is a Senior Research Associate and Fellow of Downing College Cambridge. Within the lab he is responsible for the experimental research programme, his research is working to obtain evidence on the dynamic nature of nuclear receptors, include the oestrogen receptor in breast cancer tumour cells. This will provide the basis for the development of a quantitative model and facilitate our understanding of what drives this process. In the long term, the model could be used to predict the effects of perturbing components of the pathway with drugs and potentially lead to improved combinatorial treatment regimes.

Research

Publications

1. Andrew N. Holding*, Federico M. Giorgi, Amanda Donnelly, Amy E. Cullen, Luke A. Selth and Florian Markowetz (2018)
   Network Dynamics of ER-α activation reveals reprogramming of GRHL2
   Preprint. [bioRxiv]
   
   
2. Evangelia K. Papachristou, Kamal Kishore¹, Andrew N. Holding¹, … , Rasmus Siersbaek, Clive S. D’Santos and Jason S. Carroll (2018)
   A quantitative mass spectrometry-based approach to monitor the dynamics of endogenous chromatin-associated protein complexes
   Nature Communications, 9, 2311. [PubMed]
   
   
3. Michael J. Guertin, Amy E. Cullen, Florian Markowetz and Andrew N. Holding* (2018)
   Parallel Factor ChIP Provides Essential Internal Control for Quantitative Differential ChIP-Seq
   Nucleic Acids Research. 46, 12. [PubMed]
   
   
4. Andrew N. Holding* (2015)
   XL–MS: Protein cross-linking coupled with mass spectrometry
   Methods 89. 54-63. [Science Direct]
   
   
5. Jennifer Lee, ShuJing Ding, Thomas B. Walpole, Andrew N. Holding, Martin G. Montgomery, Ian M. Fearnley and John E. Walker (2015)
   Organisation of Subunits in the Membrane Domain of the Bovine F-ATPase Revealed by Covalent Cross-linking.
   J. Biol. Chem. 290. 13308-20. [PubMed]
   
   
6. Andrew N Holding,* Meindert H Lamers, Elaine Stephens and J Mark Skehel (2013)
   Hekate: software suite for the mass spectrometric analysis and three-dimensional visualization of cross-linked protein samples.
   J Proteome Res. 12. 5923-33. [PubMed]
   
   
7. Yang Liu,¹ Hannah K Salter, ¹ Andrew N Holding, Christopher M Johnson, Elaine Stephens, Peter J Lukavsky, John Wlashaw and Simon L Bullock (2013)
   Bicaudal-D uses a parallel, homodimeric coiled coil with heterotypic registry to coordinate recruitment of cargos to dynein.
   Genes Dev. 27. 1233-46. [PubMed]
   
   
8. Andrew N Holding,¹ Ana Toste Rego,¹ Helen Kent and Meindert H Lamers (2013)
   Architecture of the Pol III—clamp—exonuclease complex reveals key roles of the exonuclease subunit in processive DNA synthesis and repair.
   EMBO J. 32. 1334-1343. [Pubmed] [F1000Prime] - recommended as being of special significance.
   
   
9. Andrew N Holding* and Jonathan B Spencer. (2008)
   Investigation into the mechanism of phenolic couplings during the biosynthesis of glycopeptide antibiotics.
   ChemBioChem. 9. 2209-14. [Pubmed]
   
   
10. Helen M O’Hare, Fanglu Huang, Andrew Holding, Oliver W Choroba and Jonathan B Spencer. (2006)
    Conversion of hydroxyphenylpyruvate dioxygenases into hydroxymandelate synthases by directed evolution.
    FEBS Lett. 580. 3445-50. [Pubmed]
    
    

¹These authors contributed equally to this work.
* Corresponding author.

Oral Presentations

1. Integrative analysis of ER-α in breast cancer reveals a robust and sustained response to estra-2-diol
   Keynote speaker, Biosciences Symposium, 2018, Essex.
2. Network Dynamics of ER-α activation reveals reprogramming of GRHL2
   Applied Bioinformatics In Life Sciences, 2018, Leuven, Belgium.
3. Utilising mass spectrometry for the structural and functional analysis of complex systems in medical research
   Invited speaker, 2016, University of Essex, Colchester, UK.
4. Visualizing gene regulation: a combined proteomic and genomic approach for the structural analysis of steroid hormone receptor complexes
   Invited speaker, 4th International Congress on Analytical Proteomics, 2015, Lisbon, Portugal.
5. Bringing Proteogenomics to the study of ER+ breast cancer
   Invited speaker, 2014, MRC Cancer Unit, Cambridge, UK.
6. Interactions within the E. coli Replisome
   Mini-symposium on Biophysics, 2012, MRC Laboratory of Molecular Biology.
7. Interactions within the E. coli Replisome
   Proteomics Methods Forum, 2012, Barts Cancer Institue, London, UK.
   
8. Cross-linking in mass spectrometry
   Proteomics Methods Forum, 2010, Department of Biochemistry, University of Cambridge.
   
9. Cross-linking in mass spectrometry
   Mini-symposium on Biophysics, 2010, MRC Laboratory of Molecular Biology.
   

Poster Presentations

1. Parallel Factor ChIP Provides Essential Internal Control for Quantitative Differential ChIP-seq
   M. J. Guertin, A. E. Cullen, F. Markowetz A. N. Holding,
   Cambridge Cancer Centre Symposium, 2017, Cambridge, UK.
2. ChIP-MS/TMT: a quantative proteomic analysis of steroid hormone receptor activation.
   A. N. Holding, F. Markowetz
   64th ASMS Conference on Mass Spectrometry and Allied Topics, 2016, San Antonio, Texas, USA
3. Visualising gene regulation: A combined proteomic and genomic approach for the analysis of steroid hormone receptor complexes.
   A. N. Holding, J. C. Carroll, F. Markowetz
   29th Annual Symposium of the Protein Society, Barcelona, Spain
4. Visualising gene regulation: A combined proteomic and genomic approach for the analysis of steroid hormone receptor complexes.
   A. N. Holding, J. C. Carroll, F. Markowetz
   Cambridge Cancer Centre Symposium, 2015, Cambridge, UK.
5. Hekate – Software suite for the mass spectrometric analysis & three-dimensional visualization of cross-linked protein samples.
   A. N. Holding, Y. Liu, H. K. Salter, M. L. Lamers, S. L. Bullock, M. J. Skehel
   Postdoctoral Career Development Day, Cancer Research UK, 2014,London, UK
6. A shared binding site for different cargo adaptors in the asymmetric coiled-coil structure of the dynein co-factor Bicaudal-D
   H. K. Salter, Y. Liu, A. N. Holding, C. Johnson, E. Stephens, J. Walshaw, S. L. Bullock
   The American Society For Cell Biology Annual Meeting, 2012, San Franciso, CA, USA.
7. Resolving interactions involved in binding of the dynein co-factor Bicaudal-D (BicD) to Rab6
   Andrew N Holding, Elaine Stephens, Mark Skehel.
   EuPA/BSPR Prteomic Meeting, 2012, Glasgow, Scotland.
8. Resolving interactions involved in binding of the dynein co-factor Bicaudal-D (BicD) to Rab6
   Andrew N Holding, Elaine Stephens.
   60th ASMS Conference on Mass Spectrometry and Allied Topics, 2012, Vancouver Convention Centre, Vancouver, BC, Canada.
   
9. XL-SAXS and DNA replication
   Ana Toste Rêgo, Andrew Holding, Michal Hammel, Elaine Stephens, John Kuriyan and Meindert H. Lamers.
   Current Opinion in Structural Biology & DNA Repair, 2011, Amsterdam, The Netherlands
10. Development of arginine and guanine specific isotopically-labelled linkers for the characterisation of binding interactions by mass spectrometry
    Andrew N Holding, Elaine Stephens.
    59th ASMS Conference on Mass Spectrometry and Allied Topics, 2011, Colorado Convention Center, Denver, CO, USA.
    
11. Sdo1/Efl1 interaction site mapped by photocrosslinking
    Andrew N Holding, Nicholas Basse, Alan Warren, Elaine Stephens.
    Memorial symposium for Dr. Jonathan Spencer, 2010, University of Cambridge.
    
12. Investigation into the mechanism of phenolic couplings during the biosynthesis of glycopeptide antibiotics
    Andrew N Holding, Jonathan B Spencer.
    Zing Conference in Natural Products, 2009, Antigua.
    

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