Andrew Holding

Andrew is a Senior Research Associate and Bye-Fellow of Downing College Cambridge. Within the lab he is responsible for the experimental research programme, his research is working to obtain evidence on the dynamic nature of the oestrogen receptor in breast cancer tumour cells. This will provide the basis for the development of a quantitative model and facilitate our understanding of what drives this process. In the long term, the model could be used to predict the effects of perturbing components of the pathway with drugs and potentially lead to improved combinatorial treatment regimes.

Degrees

PhD in Chemical Biology
Studies on the biosynthesis of non-ribosomal peptides
University of Cambridge, UK

Masters in Chemistry
University of Oxford, UK

Vita

2015
Senior Research Associate
Cancer Research UK Cambridge Research Institute

2013 - 2014
Research Associate
Cancer Research UK Cambridge Research Institute

2009 - 2013
Career Development Fellow
MRC Laboratory of Molecular Biology

2005 - 2009
PhD student
Deparment of Chemistry, University of Cambridge, UK.
Advisors: Dr. J. Spencer and Dr. F. Leeper

Awards and fellowships

2015 CRUK Rising Star in Research Engagement Prized

2015 Fellow Downing College

2014 Unltd Try-It Award

2013 College Lecturer and Bye-Fellow Downing College

2012 Wellcome Trust People Award

2012 British Mass Spectrometry Society Travel Award

2012 MRC Award via the Special Awards Scheme for contributions to the MRC

2011 UnLtd Catalyst Award for Social Entrepreneurship

2009 MRC Career Development Fellowship

2005 BBSRC PhD Studentship

Professional Memberships

2015 Fellow of the Higher Education Academy

2014 Member Biochemical Society

2009 Member Royal Society of Chemistry

Andrew Holding

Andrew Holding
University of Cambridge
CRUK Cambridge Institute
Li Ka Shing Centre
Robinson Way
Cambridge, CB2 0RE, UK
e: first.last@cruk.cam.ac.uk
p: +44 (0) 1223 769 696
w: Andrew Holding

BCC logo Cambridge University

Publications

Oral Presentations

  1. Visualizing gene regulation: a combined proteomic and genomic approach for the structural analysis of steroid hormone receptor complexes
    Invited speaker, 4th International Congress on Analytical Proteomics, 2015, Lisbon, Portugal.
  2. Bringing Proteogenomics to the study of ER+ breast cancer
    Fellows' Day 2014, MRC Cancer Unit, Cambridge, UK
  3. Interactions within the E. coli Replisome
    Mini-symposium on Biophysics, 2012, MRC Laboratory of Molecular Biology.
  4. Interactions within the E. coli Replisome
    Proteomics Methods Forum, 2012, Barts Cancer Institue, London, UK.
  5. Cross-linking in mass spectrometry
    Proteomics Methods Forum, 2010, Department of Biochemistry, University of Cambridge.
  6. Cross-linking in mass spectrometry
    Mini-symposium on Biophysics, 2010, MRC Laboratory of Molecular Biology.

Poster Presentations

  1. Visualising gene reguation: A combined proetomic and genomic approach for the analysis of steroid hormone receptor complexes.
    A. N. Holding, J. C. Carroll, F. Markowetz
    29th Annual Symposium of the Protein Society, Barcelona, Spain
  2. Visualising gene reguation: A combined proetomic and genomic approach for the analysis of steroid hormone receptor complexes.
    A. N. Holding, J. C. Carroll, F. Markowetz
    Cambridge Cancer Centre Symposium, 2015, Cambridge, UK.
  3. A shared binding site for different cargo adaptors in the asymmetric coiled-coil structure of the dynein co-factor Bicaudal-D
    H. K. Salter, Y. Liu, A. N. Holding, C. Johnson, E. Stephens, J. Walshaw, S. L. Bullock
    The American Society For Cell Biology Annual Meeting, 2012, San Franciso, CA, USA.
  4. Resolving interactions involved in binding of the dynein co-factor Bicaudal-D (BicD) to Rab6
    Andrew N Holding, Elaine Stephens, Mark Skehel.
    EuPA/BSPR Proteomic Meeting, 2012, Glasgow, Scotland.
  5. Resolving interactions involved in binding of the dynein co-factor Bicaudal-D (BicD) to Rab6
    Andrew N Holding, Elaine Stephens.
    60th ASMS Conference on Mass Spectrometry and Allied Topics, 2012, Vancouver Convention Centre, Vancouver, BC, Canada.
  6. XL-SAXS and DNA replication
    Ana Toste Rêgo, Andrew Holding, Michal Hammel, Elaine Stephens, John Kuriyan and Meindert H. Lamers.
    Current Opinion in Structural Biology & DNA Repair, 2011, Amsterdam, The Netherlands
  7. Development of arginine and guanine specific isotopically-labelled linkers for the characterisation of binding interactions by mass spectrometry
    Andrew N Holding, Elaine Stephens.
    59th ASMS Conference on Mass Spectrometry and Allied Topics, 2011, Colorado Convention Center, Denver, CO, USA.
  8. Sdo1/Efl1 interaction site mapped by photocrosslinking
    Andrew N Holding, Nicholas Basse, Alan Warren, Elaine Stephens.
    Memorial symposium for Dr. Jonathan Spencer, 2010, University of Cambridge.
  9. Investigation into the mechanism of phenolic couplings during the biosynthesis of glycopeptide antibiotics
    Andrew N Holding, Jonathan B Spencer.
    Zing Conference in Natural Products, 2009, Antigua.

Publications

  1. Andrew N. Holding
    XL-MS: Protein cross-linking coupled with mass spectrometry
    Methods. 2015. [Science Direct]

  2. Jennifer Lee, ShuJing Ding, Thomas B. Walpole, Andrew N. Holding, Martin G. Montgomery, Ian M. Fearnley and John E. Walker (2015)
    Organisation of Subunits in the Membrane Domain of the Bovine F-ATPase Revealed by Covalent Cross-linking.
    J. Biol. Chem. 290. 13308-20. [PubMed]

  3. Andrew N Holding, Meindert H Lamers, Elaine Stephens, J Mark Skehel (2013)
    Hekate: software suite for the mass spectrometric analysis and three-dimensional visualization of cross-linked protein samples.
    J Proteome Res. 12. 5923-33. [PubMed]

  4. Yang Liu,1 Hannah K Salter, 1 Andrew N Holding, Christopher M Johnson, Elaine Stephens, Peter J Lukavsky, John Wlashaw, Simon L Bullock (2013)
    Bicaudal-D uses a parallel, homodimeric coiled coil with heterotypic registry to coordinate recruitment of cargos to dynein.
    Genes Dev. 27. 1233-46. [PubMed]

  5. Andrew N Holding,1 Ana Toste Rego,1 Helen Kent and Meindert H Lamers (2013)
    Architecture of the Pol III—clamp—exonuclease complex reveals key roles of the exonuclease subunit in processive DNA synthesis and repair.
    EMBO J. 32. 1334-1343. [Pubmed] [F1000Prime] - recommended as being of special significance.

  6. Holding AN, Spencer JB. (2008)
    Investigation into the mechanism of phenolic couplings during the biosynthesis of glycopeptide antibiotics.
    ChemBioChem. 9. 2209-14. [Pubmed]

  7. O'Hare HM, Huang F, Holding A, Choroba OW, Spencer JB. (2006)
    Conversion of hydroxyphenylpyruvate dioxygenases into hydroxymandelate synthases by directed evolution.
    FEBS Lett. 580. 3445-50. [Pubmed]